Camera alignment off

I installed a web camera to my Longmill that has a laser attached. The calibration and alignment went well with pictures taken well below the maximum limit. The problem is when I place a piece to be printed on the bed and put an image on it then hit frame, the frame is approx 3.25” off to the top and left. I have done the calibration and alignment 3 times and always get the same result. I am running the latest version of LB and using grbl.

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