You can, sort of, but it’s not really recommended to use the ‘Start’ button for Ruida controlled machines because ‘Start’ can on occasion screw up, so typically the Start button would be disabled by Thunder Laser if they helped set up your software.
You can override that setting for your Device by toggling the ‘Disable Start button’ setting as shown in the image below.
A better option if you really want to start the job from LightBurn, is to use Alt+Shift+S or hold down the Shift key as you click on ‘Send’ so the job auto-starts only after it has fully transferred to the machine.
