Hi Chris,
Exactly how far out is this misalignment?
Immediately after the alignment, additional marking on the same material should match up almost perfectly with the camera image.
I suggest you re-home the machine, mark a test shape while in ‘Absolute Coords’ mode , then in the ‘Camera Control’ window, ‘Update Overlay’ and use the X and Y-Shift controls to realign the overlay image of the burn marks with the test shape and then ‘Save Settings’.
Follow this forum link for other common reasons the alignment can be out.
If the material thickness changed that could have messed with the alignment. The ability to raise or lower your laser bed provides a means for keeping the material surface in the same place relative to the camera. Some lasers have an autofocus sensor which will automatically handle this.