Where do you have the Machine Origin set?
Laser Origin and homing:
The origin setting is where the ‘zero’ point of your X & Y axis meet. If you get this wrong, you can change it later in the Device Settings page. This setting also controls the orientation of the output - if it’s wrong, the output from your laser may be mirrored or upside down.
… If you have a DSP controller, like Ruida, Trocen, or TopWisdom, common in larger CO2 lasers with metal cabinets and LCD displays, the origin corner will be the corner that the laser head seeks out when you power it up.
This is the origin corner or 0,0 location for your laser. If you have a GCode based system, this is almost always at the front left, regardless of the location of your limit switches.
If you have a DSP laser, like Ruida or Trocen, the origin is usually where the limit switches are placed, and will be the corner the laser seeks when powered up.
If your laser output is mirrored horizontally or vertically, move the dot to the opposite corner, horizontally or vertically, depending on the direction that the output is mirrored, and that will correct it. Device Settings - LightBurn Software Documentation