You need to change the Device Origin. Here, we cover this subject in the documentation, where we say,
“If your laser output is mirrored horizontally or vertically, move the dot to the opposite corner, horizontally or vertically, depending on the direction that the output is mirrored, and that will correct it.”
The documentation is always a good resource worth review as we cover many of the things commonly asked. https://lightburnsoftware.github.io/NewDocs/DeviceSettings.html
Origin
This is the origin corner or 0,0 location for your laser. If you have a GCode based system, this is almost always at the front left, regardless of the location of your limit switches.
If you have a DSP laser, like Ruida or Trocen, the origin is usually where the limit switches are placed, and will will be the corner the laser seeks when powered up.
If your laser output is mirrored horizontally or vertically, move the dot to the opposite corner, horizontally or vertically, depending on the direction that the output is mirrored, and that will correct it.