Yes. If you use “User Origin” mode, it means it’s going to start from a location you set when you click that button. If you use “Current Position” mode, it will start from exactly where you have it placed when you click “Start”. The User Origin mode is more useful for things you might need to stop and re-start, or if you need to move the laser out of the way for something and then be able to get back to that known starting point, but you need to remember to set it.
Current Position will run exactly from where the machine is, but if you have to stop it, the only way to get back to that exact starting point is to know the coordinate you were at, or manually line it up again, which can be cumbersome.