You misaligned the piece in such way that the laser can’t reach it. Either it crashes into the frame (ok, I guess you would notice that), or some cabling like the air assist hose is hindering the axis from moving correctly
You did not fix the laser cable correctly, which causes the cable to loose contact once it’s in a certain position. You can test by turning on the fire function and twist and bend the cable. Make sure it’s fixed to the frame, the cable at the connector should not move at all during movements of the laser head.
Some comments on this: Guide to mechanical adjustments and maintenance