Everything is deeply intertwingled!
Caveat: I don’t know how all those parts are supposed to fit together, nor whether the red crosses represent holes, so pay attention to catch my incorrect assumptions.
I assume all the lines in those layouts are on the nominal dimensions, so that an ideal zero-width Xacto-knife cut will fit everything together.
If you apply the correct outward (positive) kerf offset to the (black?) layer with the fish outlines in the first screenshot, then their posts come out exactly at the nominal width. The notches (slots?) in the black round bases (?) also cut to the nominal size, so the tabs should fit into the slots. In both cases, the kerf offset moves the beam outward so the inner edge of the cut = outer boundary of the shape lies exactly along the nominal perimeter.
If you apply the same outward offset to the (blue-ish? green-ish?) layer with the notches (slots?) in the second screenshot, they also come out at the nominal size to match the nominal size of the red crosses.
However, if the crosses in the red layer in the first screenshot represent holes inside the black bases, then applying an outward kerf offset to the red layer will make the holes too large, because the beam will travel around the outside of the cross: LightBurn does not know those are holes.
If you apply an inward (negative) offset to the red layer, then the beam travels around the inside of the cross so the surrounding material has the correct size after the cross drops out.
Instead, you can put the crosses on the black layer, in which case LightBurn knows they are holes by counting perimeter crossings from outside the layout into the closed shape:
- Odd number of crossings = outside perimeter = object
- Even number of crossings = inside perimeter = hole
So, yes, applying a kerf to a layer means everything changes: it’s your responsibility to know which way they’ll change. You should definitely practice on cheap material until you’re sure you understand what’s going on and, even then, expect a few surprises.